Functional variants of TIM-3/HAVCR2 3'UTR in lymphoblastoid cell lines

Feifei Pu; Fengxia Chen; Zhicai Zhang; Jing Feng; Ping Xia

doi:10.4155/fsoa-2017-0121

Functional variants of TIM-3/HAVCR2 3'UTR in lymphoblastoid cell lines

Future Sci OA. 2018 Mar 15;4(5):FSO298. doi: 10.4155/fsoa-2017-0121. eCollection 2018 Jun.

Authors

Feifei Pu^{1

1}, Fengxia Chen^{2

2}, Zhicai Zhang^{3

3}, Jing Feng^{1

1}, Ping Xia^{1

1}

Affiliations

¹ Department of Orthopedics, Wuhan No.1 Hospital, Wuhan Integrated TCM & Western Medicine Hospital, Wuhan, Hubei 430022, PR China.
² Department of Medical Oncology, General Hospital of The Yangtze River Shipping, Wuhan, Hubei 430022, PR China.
³ Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan, Hubei 430022, PR China.

Abstract

Aim: Variants of TIM-3/HAVCR2 3'UTR miRNA binding sites are significantly associated with cancer; however, roles in post-transcriptional regulation have not been elucidated.

Methods: The regulatory and coding region single nucleotide polymorphisms (SNPs) of TIM-3/HAVCR2 were identified using an online database. Single nucleotide polymorphism Function Prediction was used to predict potential functional relevance of miRNA binding sites.

Results: The analysis indicated rs9313439, rs4704846, rs3087616 and rs1036199 affect possible miRNA binding sites in TIM-3/HAVCR2 3'UTR. We used additional data on genotypes and limited minor allele frequency >5% in the HapMap populations. Only rs3087616 and rs4704846 were significantly associated with TIM-3/HAVCR2.

Conclusion: Both rs3087616 and rs4704846 could be putative variants mediating post-transcriptional regulation of the TIM-3/HAVCR2. Deeper understanding of how 3'UTR variants influence the activity by TIM-3/HAVCR2 for therapy against cancer.

Keywords: TIM-3/HAVCR2; genetic; miRNA; polymorphism; variant.