Macrodiscs Comprising SMALPs for Oriented Sample Solid-State NMR Spectroscopy of Membrane Proteins

Jasmina Radoicic; Sang Ho Park; Stanley J Opella

doi:10.1016/j.bpj.2018.05.024

Macrodiscs Comprising SMALPs for Oriented Sample Solid-State NMR Spectroscopy of Membrane Proteins

Biophys J. 2018 Jul 3;115(1):22-25. doi: 10.1016/j.bpj.2018.05.024. Epub 2018 Jun 15.

Authors

Jasmina Radoicic¹, Sang Ho Park¹, Stanley J Opella²

Affiliations

¹ Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California.
² Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California. Electronic address: sopella@ucsd.edu.

Abstract

Macrodiscs, which are magnetically alignable lipid bilayer discs with diameters of >30 nm, were obtained by solubilizing protein-containing liposomes with styrene-maleic acid copolymers. Macrodiscs provide a detergent-free phospholipid bilayer environment for biophysical and functional studies of membrane proteins under physiological conditions. The narrow resonance linewidths observed from membrane proteins in styrene-maleic acid macrodiscs advance structure determination by oriented sample solid-state NMR spectroscopy.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Lipid Bilayers / chemistry*
Magnets / chemistry
Maleates / chemistry*
Membrane Proteins / chemistry*
Nuclear Magnetic Resonance, Biomolecular*
Styrene / chemistry*

Substances

Lipid Bilayers
Maleates
Membrane Proteins
Styrene
maleic acid

Abstract

Publication types

MeSH terms

Substances

Grants and funding