A naked mole rat iPSC line expressing drug-inducible mouse pluripotency factors developed from embryonic fibroblasts

Sang-Goo Lee; Aleksei E Mikhalchenko; Sun Hee Yim; Vadim N Gladyshev

doi:10.1016/j.scr.2018.06.010

A naked mole rat iPSC line expressing drug-inducible mouse pluripotency factors developed from embryonic fibroblasts

Stem Cell Res. 2018 Aug:31:197-200. doi: 10.1016/j.scr.2018.06.010. Epub 2018 Jun 20.

Authors

Sang-Goo Lee¹, Aleksei E Mikhalchenko², Sun Hee Yim¹, Vadim N Gladyshev³

Affiliations

¹ Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
² Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA; Center for Data-Intensive Biomedicine and Biotechnology, Skolkovo Institute of Science and Technology, Moscow 143028, Russia.
³ Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA. Electronic address: vgladyshev@rics.bwh.harvard.edu.

PMID: 30107334
DOI: 10.1016/j.scr.2018.06.010

Abstract

Naked mole rats (NMRs, Heterocephalus glaber) are long-lived, cancer-resistant rodents. Here, we report the development of an induced pluripotent stem cell (iPSC) line generated from immortalized NMR embryonic fibroblasts transduced with a doxycycline-inducible mouse OSKM polycistronic vector. This iPSC line was shown to express pluripotency-associated markers, form embryoid bodies, differentiate in vitro to the derivatives of three germ layers, and exhibit normal karyotype. The ability of iPSCs to differentiate in vivo was supported by the contribution to interspecific chimera upon injection into mouse blastocysts. This NMR iPSC line may be a useful tool in cancer and aging research.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cellular Reprogramming
Fibroblasts / metabolism*
Induced Pluripotent Stem Cells / metabolism*
Mice
Rats