Abstract
Allelic exclusion is a vital mechanism for the generation of monospecificity to foreign Ags in B and T lymphocytes. In this study, we developed a high-throughput barcoded method to simultaneously analyze the VDJ recombination status of both mouse TCR-β alleles in hundreds of single cells using next-generation sequencing.
Copyright © 2018 by The American Association of Immunologists, Inc.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Alleles
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Animals
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B-Lymphocytes / immunology*
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Gene Rearrangement, beta-Chain T-Cell Antigen Receptor*
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Genes, T-Cell Receptor beta / genetics*
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High-Throughput Nucleotide Sequencing
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Mice
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Mice, Inbred C57BL
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Receptors, Antigen, T-Cell, alpha-beta / genetics*
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Single-Cell Analysis
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T-Lymphocytes / immunology*
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V(D)J Recombination
Substances
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Receptors, Antigen, T-Cell, alpha-beta