CRISPR-Based Technologies: Impact of RNA-Targeting Systems

Michael P Terns

doi:10.1016/j.molcel.2018.09.018

CRISPR-Based Technologies: Impact of RNA-Targeting Systems

Mol Cell. 2018 Nov 1;72(3):404-412. doi: 10.1016/j.molcel.2018.09.018.

Author

Michael P Terns¹

Affiliation

¹ Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USA; Department of Genetics, University of Georgia, Athens, GA 30602, USA; Department of Microbiology, University of Georgia, Athens, GA 30602, USA. Electronic address: mterns@uga.edu.

Abstract

DNA-targeting CRISPR-Cas systems, such as those employing the RNA-guided Cas9 or Cas12 endonucleases, have revolutionized our ability to predictably edit genomes and control gene expression. Here, I summarize information on RNA-targeting CRISPR-Cas systems and describe recent advances in converting them into powerful and programmable RNA-binding and cleavage tools with a wide range of novel and important biotechnological and biomedical applications.

Keywords: CRISPR; Cas10; Cas13; Cas9; RNA editing; RNA targeting; Type III CRISPR-Cas systems; Type VI CRISPR-Cas systems; gene editing.

Publication types

Research Support, N.I.H., Extramural
Review

MeSH terms

Animals
CRISPR-Cas Systems / genetics*
Clustered Regularly Interspaced Short Palindromic Repeats / genetics
DNA
Gene Editing / methods*
Genetic Engineering / methods*
Humans
RNA / genetics
RNA, Guide, CRISPR-Cas Systems / genetics

Substances

RNA, Guide, CRISPR-Cas Systems
RNA
DNA

Grants and funding

R35 GM118160/GM/NIGMS NIH HHS/United States