Comparison of lung clearance index determined by washout of N2 and SF6 in infants and preschool children with cystic fibrosis

Abstract

Background: Multiple-breath washout (MBW) has been shown to detect early impairment of lung function in children with cystic fibrosis (CF). Nitrogen (N₂) or sulfur hexafluoride (SF₆) can be used as tracer gas for MBW. Recent data indicated higher lung clearance index (LCI) values measured with N₂-MBW than concurrent SF₆-MBW in older children and adults, however, a comparison in infants and younger children, as well as to other outcome measures of CF lung disease is pending.

Methods: N₂- and SF₆-MBW were performed consecutively in 31 sedated infants and preschool children with CF (mean age, 2.3 ± 0.8 years) and 20 controls (mean age, 2.3 ± 1.1 years) using the Exhalyzer D system. Children with CF also underwent chest magnetic resonance imaging (MRI).

Results: Mean difference (95% CI) in LCI between N₂- and SF₆-MBW was 1.1 ± 0.4 (0.9 to 1.3) in controls and 2.1 ± 1.9 (1.4 to 2.8) in CF. Agreement between N₂- and SF₆-LCI was poor in children with CF. N₂-LCI and SF₆-LCI correlated with MRI, however N₂-LCI showed a higher concordance with MRI than SF₆-LCI. The absolute difference between N₂- and SF₆-LCI values increased with the severity of CF lung disease as determined by MRI scores.

Conclusion: N₂-LCI values were higher than SF₆-LCI values in infants and preschool children with CF and controls. Better concordance of N₂-LCI than SF₆-LCI with chest MRI scores point towards of a higher sensitivity of N₂-LCI to detect early lung disease in children with CF.

Keywords: Clinical trial endpoint; Early CF lung disease; Infant lung function test; Lung clearance index; Magnetic resonance imaging; Multiple breath washout.