Abstract
Appropriate immune responses require a fine balance between immune activation and attenuation. NLRC3, a non-inflammasome-forming member of the NLR innate immune receptor family, attenuates inflammation in myeloid cells and proliferation in epithelial cells. T lymphocytes express the highest amounts of Nlrc3 transcript where its physiologic relevance is unknown. We show that NLRC3 attenuated interferon-γ and TNF expression by CD4+ T cells and reduced T helper 1 (Th1) and Th17 cell proliferation. Nlrc3-/- mice exhibited increased and prolonged CD4+ T cell responses to lymphocytic choriomeningitis virus infection and worsened experimental autoimmune encephalomyelitis (EAE). These functions of NLRC3 were executed in a T-cell-intrinsic fashion: NLRC3 reduced K63-linked ubiquitination of TNF-receptor-associated factor 6 (TRAF6) to limit NF-κB activation, lowered phosphorylation of eukaryotic translation initiation factor 4E-binding protein 1 (4E-BP1), and diminished glycolysis and oxidative phosphorylation. This study reveals an unappreciated role for NLRC3 in attenuating CD4+ T cell signaling and metabolism.
Keywords:
CD4(+) T cell response; EAE; LCMV infection; NLRC3; NOD-like receptors; T cell receptor signaling; autoimmunity; inflammatory pathways.
Copyright © 2018 Elsevier Inc. All rights reserved.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing
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Animals
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Autoimmunity / genetics
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Autoimmunity / immunology*
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Carrier Proteins / genetics
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Carrier Proteins / immunology
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Carrier Proteins / metabolism
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Cell Cycle Proteins
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Encephalomyelitis, Autoimmune, Experimental / genetics
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Encephalomyelitis, Autoimmune, Experimental / immunology*
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Eukaryotic Initiation Factors
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Humans
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Immunity, Innate / genetics
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Immunity, Innate / immunology*
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Intercellular Signaling Peptides and Proteins / genetics
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Intercellular Signaling Peptides and Proteins / immunology*
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Intercellular Signaling Peptides and Proteins / metabolism
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Lymphocytic Choriomeningitis / genetics
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Lymphocytic Choriomeningitis / immunology*
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Lymphocytic Choriomeningitis / microbiology
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Lymphocytic choriomeningitis virus / immunology*
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Lymphocytic choriomeningitis virus / physiology
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Mice, Inbred C57BL
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Mice, Knockout
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NF-kappa B / immunology
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NF-kappa B / metabolism
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Phosphoproteins / genetics
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Phosphoproteins / immunology
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Phosphoproteins / metabolism
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TNF Receptor-Associated Factor 6 / genetics
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TNF Receptor-Associated Factor 6 / immunology
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TNF Receptor-Associated Factor 6 / metabolism
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Th1 Cells / immunology
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Th1 Cells / metabolism
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Th17 Cells / immunology
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Th17 Cells / metabolism
Substances
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Adaptor Proteins, Signal Transducing
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Carrier Proteins
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Cell Cycle Proteins
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Eif4ebp1 protein, mouse
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Eukaryotic Initiation Factors
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Intercellular Signaling Peptides and Proteins
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NF-kappa B
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NLRC3 protein, mouse
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Phosphoproteins
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TNF Receptor-Associated Factor 6