First Genome Sequence of Newcastle Disease Virus of Genotype VIIi from Jordan

Mustafa Ababneh; Helena L Ferreira; Mohammad Khalifeh; David L Suarez; Claudio L Afonso

doi:10.1128/MRA.01136-18

First Genome Sequence of Newcastle Disease Virus of Genotype VIIi from Jordan

Microbiol Resour Announc. 2018 Dec 13;7(23):e01136-18. doi: 10.1128/MRA.01136-18. eCollection 2018 Dec.

Authors

Mustafa Ababneh¹, Helena L Ferreira^{2

3}, Mohammad Khalifeh¹, David L Suarez², Claudio L Afonso²

Affiliations

¹ Department of Basic Medical Veterinary Sciences, Jordan University of Science & Technology, Irbid, Jordan.
² U.S. National Poultry Research Center, Southeast Poultry Research Laboratory, Athens, Georgia, USA.
³ Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of São Paulo (FZEA-USP), Pirassununga, São Paulo, Brazil.

Abstract

Newcastle disease virus (NDV) was detected by reverse transcriptase PCR (RT-PCR) from total RNA isolated from a chicken spleen of a backyard flock in Jordan. The complete coding genome sequence of NDV/chicken/Jordan/J11-spleen/2018 was obtained with MiSeq (Illumina) sequencing. Phylogenetic analysis of the concatenated coding sequences classified the virus as class II subgenotype VIIi.