Generation of seven induced pluripotent stem cell lines from neonates of different ethnic backgrounds

Yingnan Yin; Andrew J Petersen; Cheryl Soref; William D Richards; Tenneille Ludwig; Seth Taapken; Erich Berndt; Su-Chun Zhang; Anita Bhattacharyya

doi:10.1016/j.scr.2018.101365

Generation of seven induced pluripotent stem cell lines from neonates of different ethnic backgrounds

Stem Cell Res. 2019 Jan:34:101365. doi: 10.1016/j.scr.2018.101365. Epub 2018 Dec 20.

Authors

Yingnan Yin¹, Andrew J Petersen¹, Cheryl Soref¹, William D Richards¹, Tenneille Ludwig², Seth Taapken², Erich Berndt¹, Su-Chun Zhang³, Anita Bhattacharyya⁴

Affiliations

¹ Waisman Center, University of Wisconsin, Madison, WI, USA.
² WiCell Research Institute, Madison, WI, USA.
³ Waisman Center, University of Wisconsin, Madison, WI, USA; Departments of Neuroscience, Neurology, School of Medicine and Public Health, University of Wisconsin, Madison, WI, USA.
⁴ Waisman Center, University of Wisconsin, Madison, WI, USA; Department of Cell and Regenerative Biology, School of Medicine and Public Health, University of Wisconsin, Madison, WI, USA. Electronic address: bhattacharyy@waisman.wisc.edu.

Abstract

Seven human induced pluripotent stem cell (iPSC) lines were generated from fibroblasts from three neonatal individuals using non-integrative reprogramming. Most control iPSCs are derived from adults, so these iPSCs meet the need for control iPSCs from young individuals. Donors were from different ethnicities and these lines provide unique genetic profiles. All iPSCs have normal karyotypes, express stem cell markers, and exhibit pluripotency, as assessed by capacity to differentiate into three germ layers. These lines are valuable to study human development, as age-matched controls for disorder-specific iPSCs, and as platforms for gene editing to control for age and ethnicity.

Keywords: Control iPSCs; Neonatal; Unaffected.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Animals
Cell Culture Techniques / methods*
Cell Line
Ethnicity*
Female
Humans
Infant, Newborn
Male
Mice

Grants and funding

U54 HD090256/HD/NICHD NIH HHS/United States