The long non-coding RNA LINC00941 and SPRR5 are novel regulators of human epidermal homeostasis

EMBO Rep. 2019 Feb;20(2):e46612. doi: 10.15252/embr.201846612. Epub 2019 Jan 8.

Abstract

Several long non-coding RNAs (lncRNAs) act as regulators of cellular homeostasis; however, few of these molecules were functionally characterized in a mature human tissue environment. Here, we report that the lncRNA LINC00941 is a crucial regulator of human epidermal homeostasis. LINC00941 is enriched in progenitor keratinocytes and acts as a repressor of keratinocyte differentiation. Furthermore, LINC00941 represses SPRR5, a previously uncharacterized molecule, which functions as an essential positive regulator of keratinocyte differentiation. Interestingly, 54.8% of genes repressed in SPRR5-deficient epidermal tissue are induced in LINC00941-depleted organotypic epidermis, suggesting a common mode of action for both molecules.

Keywords: LINC00941; SPRR5; differentiation; epidermis; long non‐coding RNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Differentiation / genetics
  • Cornified Envelope Proline-Rich Proteins / genetics*
  • Cornified Envelope Proline-Rich Proteins / metabolism
  • Epidermal Cells / metabolism*
  • Epidermis / metabolism*
  • Gene Knockout Techniques
  • Homeostasis*
  • Humans
  • Keratinocytes / cytology
  • Keratinocytes / metabolism
  • RNA, Long Noncoding / genetics*
  • Transcription, Genetic

Substances

  • Cornified Envelope Proline-Rich Proteins
  • RNA, Long Noncoding