Real-time imaging of senescence in tumors with DNA damage

Sci Rep. 2019 Feb 14;9(1):2102. doi: 10.1038/s41598-019-38511-z.

Abstract

Detection of cellular senescence is important not only in the study of senescence in various biological systems, but also in various practical applications such as image-guided surgical removal of senescent cells, as well as the monitoring of drug-responsiveness during cancer therapies. Due to the lack of suitable imaging probes for senescence detection, particularly in living subjects, we have developed an activatable near-infrared (NIR) molecular probe with far-red excitation, NIR emission, and high "turn-on" ratio upon senescence-associated β-galactosidase (SABG) activation. We present here the first successful demonstration of NIR imaging of DNA damage-induced senescence both in vitro and in human tumor xenograft models.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis
  • Cell Proliferation
  • Cellular Senescence*
  • Colonic Neoplasms / genetics
  • Colonic Neoplasms / pathology*
  • DNA Damage*
  • Female
  • Fluorescent Dyes
  • Humans
  • Image Processing, Computer-Assisted / methods*
  • Mice
  • Mice, Nude
  • Microscopy, Fluorescence / methods*
  • Tumor Cells, Cultured
  • Xenograft Model Antitumor Assays

Substances

  • Fluorescent Dyes