Lactobacillus zhachilii sp. nov., a lactic acid bacterium isolated from Zha-Chili

Abstract

Strain HBUAS52074^T is a Gram-positive staining, aerobic bacterium that was isolated from Zha-Chili, a traditional fermented food made in China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain HBUAS52074^T is a member of the genus Lactobacillus and closely related to Lactobacillus nantensis DSM 16982^T (98.9 %), Lactobacillus heilongjiangensis DSM 28069^T (98.8 %), Lactobacillus formosensis NBRC 1095009^T (98.6 %), Lactobacillus futsaii JCM 17355^T (98.5 %), Lactobacillus farciminis KCTC 3681^T (98.5 %), Lactobacillus musae NBRC 112868^T (98.5 %) and Lactobacillus crustorum LMG 23699^T (98.4 %). The DNA G+C content is 36.3 mol%. The major cellular fatty acids are C16 : 0 (28.2 %), C18 : 1ω9c (30.5 %) and summed feature 7 (C19 : 1ω6c, and/or C19 : 1ω7c; 14.9 %). Average nucleotide identity and DNA-DNA hybridization (GGDC) values based on genomic comparisons between HBUAS52074^T and related type species showed that the bacterium was significantly different from its closest relatives. Using polyphasic taxonomic analysis, we have shown that strain HBUAS52074^T is a new species in the genus Lactobacillus, for which we propose the name Lactobacilluszhachilii sp. nov. The type strain is HBUAS52074^T (=GDMCC 1.1417^T=KCTC 21106^T).

Keywords: Lactobacillus zhachilii; Zha-Chili; phylogenetic analysis.