Mitochondria are involved in a variety of cellular metabolic processes and their functions are regulated by intrinsic and extrinsic stimuli. Recent studies have revealed functional diversity and importance of mitochondria in many cellular processes, including the innate immune response. This study evaluated the specific response and proteomic changes in host Caenorhabditis elegans mitochondria during Pseudomonas aeruginosa PAO1 infection. We performed an inclusive approach to determine the C. elegans mitochondria proteome. The protein fractions of mitochondria were analysed by tandem LC-MS/MS, 129 differentially regulated proteins were identified, indicating an involvement of various mitochondrial processes. The several known components of the oxidative phosphorylation (OXPHOS) machinery, the tricarboxylic acid (TCA) cycle, mitochondrial unfolded protein response (UPRmt) and stable mitochondria-encoded proteins were found to be differentially expressed. Our results in-depth provide new horizons for mitochondria-associated protein functions and the classification of mitochondrial diseases during host-pathogen interaction.
Keywords: Caenorhabditis elegans; Infection; Mitochondrial proteomic analysis; Pseudomonas aeruginosa PAO1.
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