Highly efficient transgene-free targeted mutagenesis and single-stranded oligodeoxynucleotide-mediated precise knock-in in the industrial microalga Euglena gracilis using Cas9 ribonucleoproteins

Toshihisa Nomura; Komaki Inoue; Yukiko Uehara-Yamaguchi; Koji Yamada; Osamu Iwata; Kengo Suzuki; Keiichi Mochida

doi:10.1111/pbi.13174

Highly efficient transgene-free targeted mutagenesis and single-stranded oligodeoxynucleotide-mediated precise knock-in in the industrial microalga Euglena gracilis using Cas9 ribonucleoproteins

Plant Biotechnol J. 2019 Nov;17(11):2032-2034. doi: 10.1111/pbi.13174. Epub 2019 Jun 11.

Authors

Toshihisa Nomura^{1

2}, Komaki Inoue¹, Yukiko Uehara-Yamaguchi¹, Koji Yamada^{2

3}, Osamu Iwata^{2

3}, Kengo Suzuki^{2

3}, Keiichi Mochida^{1

2

4

5

6}

Affiliations

¹ RIKEN Center for Sustainable Resource Science, Tsurumi-ku, Yokohama, Japan.
² RIKEN Baton Zone Program, Tsurumi-ku, Yokohama, Japan.
³ euglena Co., Ltd., Tokyo, Japan.
⁴ Kihara Institute for Biological Research, Yokohama City University, Totsuka-ku, Yokohama, Japan.
⁵ Graduate School of Nanobioscience, Yokohama City University, Tsurumi-ku, Yokohama, Japan.
⁶ Institute of Plant Science and Resources, Okayama University, Kurashiki, Okayama, Japan.

No abstract available

Keywords: Euglena gracilis; CRISPR/Cas9; Microalgae; genome editing; knock-in; ribonucleoproteins; single-stranded oligodeoxynucleotides; transgene free.

Publication types

Editorial

MeSH terms

CRISPR-Cas Systems*
Euglena gracilis / growth & development*
Gene Editing*
Gene Knock-In Techniques
Microalgae / genetics*
Mutagenesis*
Oligodeoxyribonucleotides
Ribonucleoproteins

Substances

Oligodeoxyribonucleotides
Ribonucleoproteins