Erythrobacter marisflavi sp. nov., isolated from isolated from estuary water

Abstract

A Gram-stain-negative, aerobic, non-motile and coccoid-, ovoid- or rod-shaped bacterial strain, designated KEM-5^T, was isolated from water sampled at an estuary environment on the Yellow Sea, Republic of Korea. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain KEM-5^T fell within the clade comprising the type strains of Erythrobacter species. Strain KEM-5^T exhibited 16S rRNA gene sequence similarities of 97.01-97.66 % to the type strains of E.rythrobacter citreus, E.rythrobacter seohaensis and E.rythrobacter pelagi and of 94.18-96.95 % to the type strains of the other Erythrobacter species. The genomicaverage nucleotide identity values of strain KEM-5^T with a non-type strain (LAMA 915) of E. citreus and E. seohaensis SW-135^T were 76.04 and 74.98 %, respectively. Mean DNA-DNA relatedness values between strain KEM-5^T and the type strains of E. citreus, E. seohaensis and E. pelagi were 10-18 %. Strain KEM-5^T contained Q-10 as the predominant ubiquinone and C_{18 : 1} ω7c, summed feature 3 (C_{16 : 1}ω7c and/or C_{16 : 1}ω6c), C_{16 : 0} and C_{17 : 1}ω6c as the major fatty acids. The major polar lipids of strain KEM-5^T were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and sphingoglycolipid. The DNA G+C content of strain KEM-5^T was 62.4 mol%. Distinguishing phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain KEM-5^T is separated from recognized Erythrobacter species. On the basis of the data presented here, strain KEM-5^T is considered to represent a novel species of the genus Erythrobacter, for which the name Erythrobacter marisflavi sp. nov. is proposed. The type strain is KEM-5^T (=KACC 19865^T=KCTC 62896^T=NBRC 113546^T).

Keywords: Erythrobacter marisflavi; estuary environment; novel species; polyphasic taxonomy.