A genetically encodable cell-type-specific protein synthesis inhibitor

Nat Methods. 2019 Aug;16(8):699-702. doi: 10.1038/s41592-019-0468-x. Epub 2019 Jul 15.

Abstract

Chemical inhibitors have revealed requirements for protein synthesis that drive cellular plasticity. We developed a genetically encodable protein synthesis inhibitor (gePSI) to achieve cell-type-specific temporal control of protein synthesis. Controlled expression of the gePSI in neurons or glia resulted in rapid, potent and reversible cell-autonomous inhibition of protein synthesis. Moreover, gePSI expression in a single neuron blocked the structural plasticity induced by single-synapse stimulation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cells, Cultured
  • Genetic Engineering*
  • HeLa Cells
  • Hippocampus / cytology
  • Hippocampus / drug effects
  • Hippocampus / metabolism*
  • Humans
  • Neuroglia / cytology
  • Neuroglia / drug effects
  • Neuroglia / metabolism
  • Neuronal Plasticity / drug effects*
  • Neurons / cytology
  • Neurons / drug effects
  • Neurons / metabolism*
  • Protein Biosynthesis*
  • Protein Synthesis Inhibitors / chemistry
  • Protein Synthesis Inhibitors / pharmacology*
  • Rats
  • Synapses / drug effects
  • Synapses / metabolism*

Substances

  • Protein Synthesis Inhibitors