Abstract
Chemical inhibitors have revealed requirements for protein synthesis that drive cellular plasticity. We developed a genetically encodable protein synthesis inhibitor (gePSI) to achieve cell-type-specific temporal control of protein synthesis. Controlled expression of the gePSI in neurons or glia resulted in rapid, potent and reversible cell-autonomous inhibition of protein synthesis. Moreover, gePSI expression in a single neuron blocked the structural plasticity induced by single-synapse stimulation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cells, Cultured
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Genetic Engineering*
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HeLa Cells
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Hippocampus / cytology
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Hippocampus / drug effects
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Hippocampus / metabolism*
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Humans
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Neuroglia / cytology
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Neuroglia / drug effects
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Neuroglia / metabolism
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Neuronal Plasticity / drug effects*
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Neurons / cytology
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Neurons / drug effects
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Neurons / metabolism*
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Protein Biosynthesis*
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Protein Synthesis Inhibitors / chemistry
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Protein Synthesis Inhibitors / pharmacology*
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Rats
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Synapses / drug effects
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Synapses / metabolism*
Substances
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Protein Synthesis Inhibitors