Background: We have previously reported clinical efficacy with a novel form of photobiomodulation-a biophotonic platform inducing fluorescent light energy (FLE)-in both disease-affected and healthy skin; however, the cellular mechanisms remain largely unknown. Objective: This study investigated the cellular mechanism of action of FLE on key skin and immune cells. Methods: We examined the effects of FLE on the clinical presentation of inflammation in a representative patient with acne vulgaris. The effect of FLE and an FLE-mimicking control lamp on collagen production from primary human dermal fibroblast (HDF) cells was assessed in the presence and absence of the proinflammatory cytokine, interferon gamma (IFN-γ). Cytokine production was assessed from HDF and human epidermal keratinocytes (HEK) exposed to M1 macrophage-conditioned media following illumination with either a blue light-emitting diode (LED) or FLE. Finally, the effects of FLE on angiogenesis were assessed in human aortic endothelial (HAE) cells. Results: FLE reduced inflammatory lesions and associated redness in the representative acne patient. Following the resolution of inflammation there was an overall enhancement of the skin's texture. FLE enhanced collagen production from nonstressed HDF cells, decreased the inflammatory profile of HDF and HEK cells, and enhanced angiogenesis in HAE cells. Conclusion: Our results suggest FLE is capable of enhancing collagen production, modulating cutaneous inflammation, and encouraging angiogenesis. While further research is required, our findings have important implications for approaches to treating inflammatory skin conditions and achieving better aesthetic outcomes.
Keywords: Acne; FLE; LED; aesthetic; angiogenesis; anti-inflammatory; biophotonics; chromophore; collagen; cytokines; fibroblasts; fluorescent light energy; inflammatory skin conditions; macrophages; photobiomodulation; rejuvenation; therapeutic.