A water-soluble AIE-active polyvalent glycocluster: design, synthesis and studies on carbohydrate-lectin interactions for visualization of Siglec distributions in living cell membranes

Chem Commun (Camb). 2019 Aug 13;55(66):9869-9872. doi: 10.1039/c9cc05008f.

Abstract

In this work, we designed and synthesized an aggregation-induced emission (AIE)-active tetraphenylethene-decorated pseudo-trisialic acid (TPE3S) and validated its high affinity for Siglecs using microscale thermophoresis techniques. TPE3S was a unique binding-on fluorescent trivalent sialocluster which was successfully utilized for the visualization of Siglecs expressed on the surface of mammalian cells.

MeSH terms

  • Animals
  • Carbohydrate Metabolism*
  • Cell Membrane / metabolism
  • Fluorescent Dyes / chemistry
  • HeLa Cells
  • Humans
  • Lectins / metabolism*
  • Magnetic Resonance Spectroscopy
  • Mass Spectrometry
  • PC12 Cells
  • Protein Binding
  • Rats
  • Sialic Acid Binding Immunoglobulin-like Lectins / metabolism*

Substances

  • Fluorescent Dyes
  • Lectins
  • Sialic Acid Binding Immunoglobulin-like Lectins