Information on cellular metabolism at the single-cell level can unravel countless biochemical process providing invaluable biomedical insight. Single-cell analysis field is at the very early stage at this moment, and all the work done so far are proof-of-principle work by early-stage researchers. In this chapter, I have outlined ten fundamental issues that are required for the development of robust single-cell metabolomics platform using mass spectrometry (MS).
Keywords: Single-cell analysis; Single-cell future directions; Single-cell mass spectrometry; Single-cell metabolomics; Single-cell omic.