Draft genome sequences for three unisolated Alnus-infective Frankia Sp+ strains, AgTrS, AiOr and AvVan, the first sequenced Frankia strains able to sporulate in-planta

Lorine Bethencourt; Florian Vautrin; Najwa Taib; Audrey Dubost; Lucia Castro-Garcia; Olivier Imbaud; Danis Abrouk; Pascale Fournier; Jérôme Briolay; Agnès Nguyen; Philippe Normand; Maria P Fernandez; Céline Brochier-Armanet; Aude Herrera-Belaroussi

doi:10.7150/jgen.35875

Draft genome sequences for three unisolated Alnus-infective Frankia Sp+ strains, AgTrS, AiOr and AvVan, the first sequenced Frankia strains able to sporulate in-planta

J Genomics. 2019 Sep 17:7:50-55. doi: 10.7150/jgen.35875. eCollection 2019.

Affiliations

¹ Univ Lyon, Université Lyon 1, CNRS, UMR5557, Ecologie Microbienne, INRA, UMR 1418, 43 bd du 11 novembre 1918, F-69622 Villeurbanne, France.
² Univ Lyon, Université Lyon 1, CNRS, UMR5558, Laboratoire de Biométrie et Biologie Évolutive, 43 bd du 11 novembre 1918, F-69622 Villeurbanne, France.
³ Univ Lyon, Université Lyon 1, DTAMB, FR 3728 BioEnviS, 43 bd du 11 novembre 1918, F-69622 Villeurbanne, France.
⁴ Biofidal, 170 av Gabriel Péri, F-69518 Vaulx-en-Velin, France.

Abstract

Actinobacteria from genus Frankia are able to form symbiotic associations with actinorhizal plants including alders. Among them, Sp+ strains are characterized by their ability to differentiate numerous sporangia inside host plant cells (unlike "Sp-" strains unable of in-planta sporulation). Here, we report the first genome sequences of three unisolated Sp+ strains: AgTrS, AiOr and AvVan obtained from Alnus glutinosa, A. incana and A. alnobetula (previously known as viridis), respectively (with genome completeness estimated at more than 98%). They represent new Frankia species based on Average Nucleotide Identity (ANI) calculations, and the smallest Alnus-infective Frankia genomes so far sequenced (~5 Mbp), with 5,178, 6,192 and 5,751 candidate protein-encoding genes for AgTrS, AiOr and AvVan, respectively.

Keywords: AgTrS; AiOr; AvVan; Frankia.