PP2A promotes ESCRT-0 complex formation on vacuolar membranes and microautophagy induction after TORC1 inactivation

Tasnuva Sharmin; Shamsul Morshed; Takashi Ushimaru

doi:10.1016/j.bbrc.2020.01.129

PP2A promotes ESCRT-0 complex formation on vacuolar membranes and microautophagy induction after TORC1 inactivation

Biochem Biophys Res Commun. 2020 Apr 9;524(3):614-620. doi: 10.1016/j.bbrc.2020.01.129. Epub 2020 Feb 4.

Authors

Tasnuva Sharmin¹, Shamsul Morshed¹, Takashi Ushimaru²

Affiliations

¹ Graduate School of Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, 422-8021, Japan.
² Graduate School of Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, 422-8021, Japan; Department of Science, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, 422-8021, Japan. Electronic address: ushimaru.takashi@shizuoka.ac.jp.

PMID: 32029270
DOI: 10.1016/j.bbrc.2020.01.129

Abstract

Deformation of vacuolar membranes mediated by endosomal sorting complex required for transport (ESCRT) is necessary for microautophagy. Target of rapamycin complex 1 (TORC1) protein kinase negatively regulates ESCRT-0 (Vps27-Hse1) recruitment onto vacuolar membranes and microautophagy induction. However, whether and how protein phosphatase regulates these events is unknown. Here, we show that the TORC1-downstream protein phosphatase PP2A-Cdc55 is important for these events after TORC1 inactivation in budding yeast. Loss of PP2A-Cdc55 compromised vacuolar localization of Hse1, but not Vps27. This study revealed that the orchestrated action of PP2A induces microautophagy upon TORC1 inactivation.

Keywords: ESCRT-0; Hse1; Microautophagy; PP2A; TORC1; Vps27.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Cycle Proteins / metabolism
Endosomal Sorting Complexes Required for Transport / metabolism*
Intracellular Membranes / metabolism*
Mechanistic Target of Rapamycin Complex 1 / metabolism*
Microautophagy*
Protein Binding
Protein Phosphatase 2 / metabolism
Receptors, Cytoplasmic and Nuclear / metabolism
Saccharomyces cerevisiae / cytology*
Saccharomyces cerevisiae / metabolism*
Saccharomyces cerevisiae Proteins / metabolism*
Vacuoles / metabolism*

Substances

CDC55 protein, S cerevisiae
Cell Cycle Proteins
Endosomal Sorting Complexes Required for Transport
Hse1 protein, S cerevisiae
Receptors, Cytoplasmic and Nuclear
Saccharomyces cerevisiae Proteins
VPS27 protein, S cerevisiae
Mechanistic Target of Rapamycin Complex 1
Protein Phosphatase 2