MitoBlue as a tool to analyze the mitochondria-lysosome communication

Sci Rep. 2020 Feb 26;10(1):3528. doi: 10.1038/s41598-020-60573-7.

Abstract

MitoBlue is a fluorescent bisamidine that can be used to easily monitor the changes in mitochondrial degradation processes in different cells and cellular conditions. MitoBlue staining pattern is exceptional among mitochondrial dyes and recombinant fluorescent probes, allowing the dynamic study of mitochondrial recycling in a variety of situations in living cells. MitoBlue is a unique tool for the study of these processes that will allow the detailed characterization of communication between mitochondria and lysosomes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 2-Naphthylamine / analogs & derivatives*
  • 2-Naphthylamine / pharmacology
  • Amidines / pharmacology*
  • Animals
  • Chick Embryo
  • Fibroblasts / cytology
  • Fibroblasts / metabolism*
  • Lysosomes / metabolism*
  • Microscopy, Fluorescence
  • Mitochondria / metabolism*

Substances

  • Amidines
  • MitoBlue
  • 2-Naphthylamine