Quantitative of progesterone using isotope dilution-matrix-assisted laser desorption ionization-time of flight mass spectrometry

Ming-Hui Yang; Han-Ping You; Hsin-Yi Wu; Yi-Ming Arthur Chen; Ying-Fong Huang; Yu-Chang Tyan

doi:10.1016/j.mex.2020.100812

Quantitative of progesterone using isotope dilution-matrix-assisted laser desorption ionization-time of flight mass spectrometry

MethodsX. 2020 Feb 20:7:100812. doi: 10.1016/j.mex.2020.100812. eCollection 2020.

Authors

Ming-Hui Yang^{1

2}, Han-Ping You³, Hsin-Yi Wu⁴, Yi-Ming Arthur Chen², Ying-Fong Huang^{3

5}, Yu-Chang Tyan^{3

6

7

8

9

10}

Affiliations

¹ National Mosquito-Borne Diseases Control Research Center, National Health Research Institutes, Miaoli, Taiwan.
² Clinical Pharmacogenomics and Pharmacoproteomics, College of Pharmacy, Taipei Medical University, Taipei, Taiwan.
³ Department of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Kaohsiung, Taiwan.
⁴ Instrumentation Center, National Taiwan University, Taipei, Taiwan.
⁵ Department of Nuclear Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan.
⁶ Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
⁷ Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung 804, Taiwan.
⁸ Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan.
⁹ Research Center for Environmental Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
¹⁰ Center for Cancer Research, Kaohsiung Medical University, Kaohsiung, Taiwan.

Abstract

A quantification assay based on isotope dilution mass spectrometry to determine the concentration of progesterone in human serum was reported. Incorporated with ¹³C₃-progesterone, serum samples were subjected to progesterone extraction and clean-up by C4 solid-phase-extraction columns and hexane-based liquid/liquid extraction, respectively. The cleaned-up serum samples were then subjected to MALDI-TOF mass spectrometry for the quantification of progesterone. In the study, the recovered progesterone concentration determined by the assay showed good robustness and constancy in comparison to conventional radioimmunologic assay. We concluded that the ¹³C₃-progesterone-based quantification assay is a robust method for the measurement of serum progesterone. Advantages of this technique includes: • This study describes a MALDI-TOF/MS method for the determination of serum progesterone. • The technique is simple and easy to apply on MALDI-TOF/MS for serum progesterone analysis. • The correlation coefficient between MALDI-TOF MS and RIA was 0.981 for serum progesterone.

Keywords: Isotope dilution; MALDI-TOF/MS; Progesterone.