Highly sensitive LC-MS/MS analysis of catecholamines in plasma

Clin Biochem. 2020 Aug:82:51-57. doi: 10.1016/j.clinbiochem.2020.03.006. Epub 2020 Mar 19.

Abstract

The catecholamines, epinephrine (E) and norepinephrine (NE) are important both as neurotransmitters and hormones, and measurement of E and NE in plasma is therefore of great interest in medical research. However, the low concentrations of E and NE in plasma require an analysis method that is both specific and sensitive. Plasma sample E and NE were extracted using solid phase extraction, and analyzed by an in-sample ion-pairing chromatography (IPC) LC-MS/MS method, that enables the ion-pairing reagent to be diverted to waste without entering the ion source of the mass spectrometer. The method was validated with good performance characteristics and the limit of quantification (LOQ) was found to be 0.20 and 0.02 nmol/L for NE and E, respectively. In conclusion, this work presents development and validation of a method for determining E and NE in plasma with a measuring range covering the entire reference interval for human plasma.

Keywords: Catecholamine; Epinephrine; In-sample Ion-pairing chromatography; Mass spectrometry; Norepinephrine; Plasma.

Publication types

  • Validation Study

MeSH terms

  • Chromatography, Liquid / methods*
  • Data Accuracy
  • Epinephrine / blood*
  • Humans
  • Neurotransmitter Agents / blood
  • Norepinephrine / blood*
  • Sensitivity and Specificity
  • Solid Phase Extraction / methods
  • Tandem Mass Spectrometry / methods*

Substances

  • Neurotransmitter Agents
  • Norepinephrine
  • Epinephrine