A new high-pressure ESI source that can be readily used for commercial API mass spectrometers in a plug-and-play manner without any modification on the ion sampling interface is introduced. The emitter can be operated at ground potential, and the positive mode electrospray is generated by applying a negative high potential to the counter electrode. A shielding electrode effectively shields the opposing electric field and improves the ion transmission. This feature facilitates the direct connection of the ESI emitter to the electrically grounded components. The application of the present ion source to the high-temperature (>100 °C) capillary liquid chromatography for high-speed separation of peptide and proteins is demonstrated using a monolithic polymeric column.
Keywords: grounded ESI emitter; high-pressure electrospray; high-speed protein separation; high-temperature capillary LC-MS; monolithic polymeric column.