Application of 3D printed tools for customized open port probe-electrospray mass spectrometry

Piotr Sosnowski; Gérard Hopfgartner

doi:10.1016/j.talanta.2020.120894

Application of 3D printed tools for customized open port probe-electrospray mass spectrometry

Talanta. 2020 Aug 1:215:120894. doi: 10.1016/j.talanta.2020.120894. Epub 2020 Mar 12.

Authors

Piotr Sosnowski¹, Gérard Hopfgartner²

Affiliations

¹ Life Sciences Mass Spectrometry, Department of Inorganic and Analytical Chemistry, University of Geneva, Quai Ernest Ansermet 24, 1211, Geneva, Switzerland.
² Life Sciences Mass Spectrometry, Department of Inorganic and Analytical Chemistry, University of Geneva, Quai Ernest Ansermet 24, 1211, Geneva, Switzerland. Electronic address: gerard.hopfgatner@unige.ch.

PMID: 32312439
DOI: 10.1016/j.talanta.2020.120894

Abstract

Three dimensional printed open port probe (3DP-OPP) and air displacement based liquid handler, were designed and optimized using fused deposition modeling (FDM) and stereolitography (SLA) 3D printing. The performance of the devices were investigated for the analysis of solid and liquid samples with electrospray ionization mass spectrometry (ESI-MS). Direct analysis in less than 1 min and without any sample preparation, enabled detection of pesticides (azoxtystrobin/imazalil) on fruits peel surface and illegal substances (MDMA/MDEA) in home-made pills. Conjunction of OPP in the overspill mode with a customized autosampler, equipped with disposable pipette tips, enables direct quantitative analysis of drugs of abuse in urine and plasma, with minimized carry-over and reduced matrix effect compared to flow injection analysis.

Keywords: 3D printing; Direct analysis; Electrospray; Fused deposition modeling; Mass spectrometry; Open port probe; Stereolithography.