Culture-Competent SARS-CoV-2 in Nasopharynx of Symptomatic Neonates, Children, and Adolescents

Emerg Infect Dis. 2020 Oct;26(10):2494-2497. doi: 10.3201/eid2610.202403. Epub 2020 Jun 30.

Abstract

Children do not seem to drive transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We isolated culture-competent virus in vitro from 12 (52%) of 23 SARS-CoV-2-infected children; the youngest was 7 days old. Our findings show that symptomatic neonates, children, and teenagers shed infectious SARS-CoV-2, suggesting that transmission from them is plausible.

Keywords: 2019 novel coronavirus disease; COVID-19; SARS-CoV-2; Switzerland; adolescents; children; coronavirus disease; neonates; respiratory diseases; severe acute respiratory syndrome coronavirus 2; viral shedding; viruses; zoonoses.

Publication types

  • Letter
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Betacoronavirus / isolation & purification
  • Betacoronavirus / physiology*
  • COVID-19
  • Cell Line
  • Child
  • Child, Preschool
  • Coronavirus Infections / diagnosis
  • Coronavirus Infections / transmission*
  • Female
  • Humans
  • Infant
  • Infant, Newborn
  • Male
  • Nasopharynx / virology*
  • Pandemics
  • Pneumonia, Viral / diagnosis
  • Pneumonia, Viral / transmission*
  • RNA, Viral / analysis*
  • SARS-CoV-2
  • Viral Load
  • Virus Cultivation
  • Virus Replication

Substances

  • RNA, Viral