Glucocorticoid receptor complexes form cooperatively with the Hsp90 co-chaperones Pp5 and FKBPs

Sci Rep. 2020 Jul 1;10(1):10733. doi: 10.1038/s41598-020-67645-8.

Abstract

The function of steroid receptors in the cell depends on the chaperone machinery of Hsp90, as Hsp90 primes steroid receptors for hormone binding and transcriptional activation. Several conserved proteins are known to additionally participate in receptor chaperone assemblies, but the regulation of the process is not understood in detail. Also, it is unknown to what extent the contribution of these cofactors is conserved in other eukaryotes. We here examine the reconstituted C. elegans and human chaperone assemblies. We find that the nematode phosphatase PPH-5 and the prolyl isomerase FKB-6 facilitate the formation of glucocorticoid receptor (GR) complexes with Hsp90. Within these complexes, Hsp90 can perform its closing reaction more efficiently. By combining chemical crosslinking and mass spectrometry, we define contact sites within these assemblies. Compared to the nematode Hsp90 system, the human system shows less cooperative client interaction and a stricter requirement for the co-chaperone p23 to complete the closing reaction of GR·Hsp90·Pp5/Fkbp51/Fkbp52 complexes. In both systems, hormone binding to GR is accelerated by Hsp90 alone and in the presence of its cofactors. Our results show that cooperative complex formation and hormone binding patterns are, in many aspects, conserved between the nematode and human systems.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Caenorhabditis elegans
  • Caenorhabditis elegans Proteins / metabolism*
  • Glycoproteins / metabolism*
  • HSP90 Heat-Shock Proteins / metabolism*
  • Humans
  • Protein Binding
  • Protein Interaction Domains and Motifs
  • Receptors, Glucocorticoid / metabolism*
  • Tacrolimus Binding Proteins / metabolism*

Substances

  • Caenorhabditis elegans Proteins
  • Glycoproteins
  • HSP90 Heat-Shock Proteins
  • Receptors, Glucocorticoid
  • tissue-factor-pathway inhibitor 2
  • Tacrolimus Binding Proteins