Abstract
Transformed mammalian cells express a unique bifunctional NAD-dependent methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase in addition to the usual NADP-dependent dehydrogenase-cyclohydrolase-synthetase. Subcellular fractionation of murine cell lines revealed that the NAD-dependent dehydrogenase activity is located predominantly in mitochondria, while the NADP-dependent trifunctional dehydrogenase appears to exist only in the cytosol of these cells. Western analysis using monospecific polyclonal antisera confirms the subcellular distribution of these two proteins.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aminohydrolases / isolation & purification
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Aminohydrolases / metabolism*
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Animals
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Carcinoma, Ehrlich Tumor / enzymology
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Cell Fractionation
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Cell Line, Transformed
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Cytosol / enzymology
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Immunoassay
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Methylenetetrahydrofolate Dehydrogenase (NADP) / isolation & purification
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Methylenetetrahydrofolate Dehydrogenase (NADP) / metabolism*
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Mice
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Mitochondria / enzymology*
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Multienzyme Complexes / isolation & purification
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Multienzyme Complexes / metabolism*
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Oxidoreductases / metabolism*
Substances
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Multienzyme Complexes
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methylene tetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase
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Oxidoreductases
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Methylenetetrahydrofolate Dehydrogenase (NADP)
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Aminohydrolases