NAD-dependent methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase in transformed cells is a mitochondrial enzyme

Biochem Biophys Res Commun. 1988 Aug 30;155(1):1-6. doi: 10.1016/s0006-291x(88)81040-4.

Abstract

Transformed mammalian cells express a unique bifunctional NAD-dependent methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase in addition to the usual NADP-dependent dehydrogenase-cyclohydrolase-synthetase. Subcellular fractionation of murine cell lines revealed that the NAD-dependent dehydrogenase activity is located predominantly in mitochondria, while the NADP-dependent trifunctional dehydrogenase appears to exist only in the cytosol of these cells. Western analysis using monospecific polyclonal antisera confirms the subcellular distribution of these two proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aminohydrolases / isolation & purification
  • Aminohydrolases / metabolism*
  • Animals
  • Carcinoma, Ehrlich Tumor / enzymology
  • Cell Fractionation
  • Cell Line, Transformed
  • Cytosol / enzymology
  • Immunoassay
  • Methylenetetrahydrofolate Dehydrogenase (NADP) / isolation & purification
  • Methylenetetrahydrofolate Dehydrogenase (NADP) / metabolism*
  • Mice
  • Mitochondria / enzymology*
  • Multienzyme Complexes / isolation & purification
  • Multienzyme Complexes / metabolism*
  • Oxidoreductases / metabolism*

Substances

  • Multienzyme Complexes
  • methylene tetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase
  • Oxidoreductases
  • Methylenetetrahydrofolate Dehydrogenase (NADP)
  • Aminohydrolases