Direct translation of climbing fiber burst-mediated sensory coding into post-synaptic Purkinje cell dendritic calcium

Abstract

Climbing fibers (CFs) generate complex spikes (CS) and Ca²⁺ transients in cerebellar Purkinje cells (PCs), serving as instructive signals. The so-called 'all-or-none' character of CSs has been questioned since the CF burst was described. Although recent studies have indicated a sensory-driven enhancement of PC Ca²⁺ signals, how CF responds to sensory events and contributes to PC dendritic Ca²⁺ and CS remains unexplored. Here, single or simultaneous Ca²⁺ imaging of CFs and PCs in awake mice revealed the presynaptic CF Ca²⁺ amplitude encoded the sensory input's strength and directly influenced post-synaptic PC dendritic Ca²⁺ amplitude. The sensory-driven variability in CF Ca²⁺ amplitude depended on the number of spikes in the CF burst. Finally, the spike number of the CF burst determined the PC Ca²⁺ influx and CS properties. These results reveal the direct translation of sensory information-coding CF inputs into PC Ca²⁺, suggesting the sophisticated role of CFs as error signals.

Keywords: cerebellum; climbing fiber; climbing fiber burst; mouse; neuroscience; purkinje cell calcium; sensory coding; two-photon calcium imaging.