Role of potato protein hydrolysate and exercise in preventing high-fat diet-induced hepatocyte apoptosis in senescence-accelerated mouse

Ho-Lin Chuang; Rathinasamy Baskaran; Cecilia Hsuan Day; Yueh-Min Lin; Chih-Chu Ho; Tsung-Jung Ho; Ray-Jade Chen; Bharath Kumar Mahalakshmi; Wei-Wen Kuo; Chih-Yang Huang

doi:10.1111/jfbc.13525

Role of potato protein hydrolysate and exercise in preventing high-fat diet-induced hepatocyte apoptosis in senescence-accelerated mouse

J Food Biochem. 2020 Dec;44(12):e13525. doi: 10.1111/jfbc.13525. Epub 2020 Oct 19.

Authors

Ho-Lin Chuang^{1

2}, Rathinasamy Baskaran³, Cecilia Hsuan Day⁴, Yueh-Min Lin⁵, Chih-Chu Ho^{1

2}, Tsung-Jung Ho⁶, Ray-Jade Chen⁷, Bharath Kumar Mahalakshmi⁸, Wei-Wen Kuo⁹, Chih-Yang Huang^{10

11

12

13

14}

Affiliations

¹ Department of Biological Science and Technology, College of Biopharmaceutical and Food Sciences, China Medical University, Taichung, Taiwan, ROC.
² HK. Zen Heart Group Biopharmaceutical Co. Limited, Wanchai, Hong Kong.
³ Department of Bioinformatics and Medical Engineering, Asia University, Taichung, Taiwan.
⁴ Department of Nursing, MeiHo University, Pingtung, Taiwan.
⁵ Department of Pathology, Changhua Christian Hospital, Changhua, Taiwan.
⁶ Department of Chinese Medicine, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Tzu Chi University, Hualien, Taiwan.
⁷ Department of Surgery, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.
⁸ Institute of Research and Development, Duy Tan University, Da Nang, Vietnam.
⁹ Department of Biological Science and Technology, China Medical University, Taichung, Taiwan.
¹⁰ Graduate Institute of Biomedical Sciences, China Medical University, Taichung, Taiwan.
¹¹ Center of General Education, Buddhist Tzu Chi Medical Foundation, Tzu Chi University of Science and Technology, Hualien, Taiwan.
¹² Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, Taiwan.
¹³ Department of Biotechnology, Asia University, Taichung, Taiwan.
¹⁴ Cardiovascular and Mitochondrial Related Disease Research Center, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Tzu Chi University of Science and Technology, Hualien, Taiwan.

PMID: 33078424
DOI: 10.1111/jfbc.13525

Abstract

Nonalcoholic fatty liver disease (NAFLD) is considered to be a serious clinical complication, which could cause significant liver dysfunction including fibrosis, cirrhosis, and cancer. Obesity could lead to NAFLD and contributes to liver disorder and related complicated liver diseases. Effect of exercise combined with alcalase treatment derived potato protein hydrolysate (APPH) on high-fat diet (HFD)-induced hepatic injury was investigated in senescence accelerated mouse-prone 8 (SAMP8) mice in the present study. Mice were divided into six groups (n = 6): Group I-Control, Group II-HFD, Group III-Exercise, Group IV-HFD + APPH, Group V-HFD + Exercise, and Group VI-HFD + Exercise + APPH. Combined APPH treatment and exercise offer better cytoprotection in HFD-induced histological changes than APPH treatment and exercise alone. Further, APPH and exercise activate the cell survival proteins PI3K/Akt and prevent FasL/FADD-mediated apoptosis in HFD fed SAMP8 mouse. APPH with swimming exercise effectively modulate HFD-induced liver damage and apoptosis in aged mice through activation of PI3K/Akt protein. PRACTICAL APPLICATIONS: Exercise training is proven to reduce the health problems associated with aging and obesity, however, intensity and duration of the exercise differs between individuals. We used integrated pharmacological and nonpharmacological approach as a therapeutic strategy for preventing HFD-induced hepatic injury in aged subjects.

Keywords: SAMP8; aging; apoptosis; high-fat diet; liver.

MeSH terms

Animals
Apoptosis
Diet, High-Fat* / adverse effects
Hepatocytes
Mice
Phosphatidylinositol 3-Kinases
Protein Hydrolysates
Solanum tuberosum*
Swimming

Substances

Protein Hydrolysates