Protocol for Measurement of Oxygen Consumption Rate In Situ in Permeabilized Cardiomyocytes

Meng Gao; Anqi Li; Yuan Qin; Bilin Liu; Guohua Gong

doi:10.1016/j.xpro.2020.100072

Protocol for Measurement of Oxygen Consumption Rate In Situ in Permeabilized Cardiomyocytes

STAR Protoc. 2020 Jul 25;1(2):100072. doi: 10.1016/j.xpro.2020.100072. eCollection 2020 Sep 18.

Authors

Meng Gao¹, Anqi Li¹, Yuan Qin^{1

2}, Bilin Liu¹, Guohua Gong¹

Affiliations

¹ Institute for Regenerative Medicine, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China.
² Department of Pharmacy, Shanghai East Hospital, Tongji University, Shanghai 200120, China.

Abstract

Analysis of mitochondrial respiration function represented by the oxygen consumption rate is necessary for assessing mitochondrial respiration function. This protocol describes steps to evaluate the respiration function of mitochondria in situ in saponin-permeabilized cardiomyocytes. In permeabilized cells, mitochondria are in a relatively integrated cellular system, and mitochondrial respiration is more physiologically relevant than isolated mitochondria. For complete details on the use and execution of this protocol, please refer to Gong et al. (2015a) and Gong et al. (2015b).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Membrane Permeability / physiology*
Cytological Techniques / methods*
Myocytes, Cardiac / metabolism*
Oxygen / analysis*
Oxygen / metabolism
Oxygen Consumption / physiology*

Substances

Oxygen