Abstract
The eukaryotic translation elongation factor 1Bγ (eEF1Bγ) is an atypical member of the glutathione transferase (GST) superfamily. Contrary to more classical GSTs having a role in toxic compound detoxification, eEF1Bγ is suggested to act as a scaffold protein, anchoring the elongation factor complex EF1B to the endoplasmic reticulum. In this study, we show that eEF1Bγ from the basidiomycete Phanerochaete chrysosporium is fully active as a glutathione transferase in vitro and undergoes conformational changes upon binding of oxidized glutathione. Using real-time analyses of biomolecular interactions, we show that GSSG allows eEF1Bγ to physically interact with other GSTs from the Ure2p class, opening new perspectives for a better understanding of the role of eEF1Bγ in cellular oxidative stress response.
Keywords:
GSSG; eEF1Bγ; glutathione transferase; time-resolved molecular dynamics.
© 2020 Federation of European Biochemical Societies.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence / genetics
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Animals
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Crystallography, X-Ray
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / ultrastructure
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Glutathione / genetics
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Glutathione Disulfide / genetics
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Glutathione Peroxidase / genetics*
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Glutathione Peroxidase / ultrastructure
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Glutathione Transferase / genetics
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Humans
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Mice
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Oxidative Stress / genetics*
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Peptide Elongation Factor 1 / genetics
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Peptide Elongation Factor 1 / ultrastructure*
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Phanerochaete / genetics*
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Phanerochaete / ultrastructure
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Prions / genetics*
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Prions / ultrastructure
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae Proteins / genetics*
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Saccharomyces cerevisiae Proteins / ultrastructure
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TEA Domain Transcription Factors
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Transcription Factors / genetics
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Transcription Factors / ultrastructure
Substances
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CAM1 protein, S cerevisiae
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DNA-Binding Proteins
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Peptide Elongation Factor 1
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Prions
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Saccharomyces cerevisiae Proteins
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TEA Domain Transcription Factors
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Tead2 protein, mouse
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Transcription Factors
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eEF1B-gamma protein, human
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Glutathione Peroxidase
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URE2 protein, S cerevisiae
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Glutathione Transferase
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Glutathione
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Glutathione Disulfide