Strand Displacement Activity of PrimPol

Int J Mol Sci. 2020 Nov 27;21(23):9027. doi: 10.3390/ijms21239027.

Abstract

Human PrimPol is a unique enzyme possessing DNA/RNA primase and DNA polymerase activities. In this work, we demonstrated that PrimPol efficiently fills a 5-nt gap and possesses the conditional strand displacement activity stimulated by Mn2+ ions and accessory replicative proteins RPA and PolDIP2. The DNA displacement activity of PrimPol was found to be more efficient than the RNA displacement activity and FEN1 processed the 5'-DNA flaps generated by PrimPol in vitro.

Keywords: FEN1; PolDIP2; PrimPol; RPA; strand displacement.

MeSH terms

  • DNA / metabolism*
  • DNA Primase / metabolism*
  • DNA-Directed DNA Polymerase / metabolism*
  • Flap Endonucleases / metabolism
  • Humans
  • Manganese / pharmacology
  • Multifunctional Enzymes / metabolism*
  • Nuclear Proteins / metabolism
  • RNA / metabolism
  • Replication Protein A / metabolism
  • Substrate Specificity / drug effects

Substances

  • Multifunctional Enzymes
  • Nuclear Proteins
  • POLDIP2 protein, human
  • Replication Protein A
  • Manganese
  • RNA
  • DNA
  • DNA Primase
  • PrimPol protein, human
  • DNA-Directed DNA Polymerase
  • Flap Endonucleases