Proteomics of Colorectal Cancer: Tumors, Organoids, and Cell Cultures-A Minireview

Philip H Lindhorst; Amanda B Hummon

doi:10.3389/fmolb.2020.604492

Proteomics of Colorectal Cancer: Tumors, Organoids, and Cell Cultures-A Minireview

Front Mol Biosci. 2020 Dec 10:7:604492. doi: 10.3389/fmolb.2020.604492. eCollection 2020.

Authors

Philip H Lindhorst¹, Amanda B Hummon^{1

2}

Affiliations

¹ Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH, United States.
² The Comprehensive Cancer Center, The Ohio State University, Columbus, OH, United States.

Abstract

Proteomics, the study of the complete protein composition of a sample, is an important field for cancer research. Changes in the proteome can serve as a biomarker of cancer or lead to the development of a targeted therapy. This minireview will focus on mass spectrometry-based proteomics studies applied specifically to colorectal cancer, particularly the variety of cancer model systems used, including tumor samples, two-dimensional (2D) and three-dimensional (3D) cell cultures such as spheroids and organoids. A thorough discussion of the application of these systems will accompany the review of the literature, as each provides distinct advantages and disadvantages for colorectal cancer research. Finally, we provide conclusions and future perspectives for the application of these model systems to cancer research as a whole.

Keywords: biomarkers; cell culture; colorectal cancer; mass spectrometry; organoids; proteomics; tumors.

Publication types

Review

Grants and funding

R01 GM110406/GM/NIGMS NIH HHS/United States