Genetically Engineered Mouse Model of Brainstem High-Grade Glioma

Fernando M Nunez; Jessica C Gauss; Flor M Mendez; Santiago Haase; Pedro R Lowenstein; Maria G Castro

doi:10.1016/j.xpro.2020.100165

Genetically Engineered Mouse Model of Brainstem High-Grade Glioma

STAR Protoc. 2020 Nov 25;1(3):100165. doi: 10.1016/j.xpro.2020.100165. eCollection 2020 Dec 18.

Authors

Fernando M Nunez^{1

2}, Jessica C Gauss^{1

2}, Flor M Mendez^{1

2}, Santiago Haase^{1

2}, Pedro R Lowenstein^{1

2}, Maria G Castro^{1

2}

Affiliations

¹ Department of Neurosurgery, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
² Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI 48109, USA.

Abstract

Brainstem gliomas are aggressive tumors that are more prevalent in pediatric patients. The location of these tumors makes them inoperable, and currently there is no effective treatment. Recent genomic data revealed the unique biology of these tumors. The following protocol provides a method to incorporate these specific genetic lesions in a mouse glioma model. Using this model, the effects of these mutations in tumor progression and response to treatments can be studied within a relevant in vivo context. For complete details on the use and execution of this protocol, please refer to Mendez et al. (2020).

Keywords: Cancer; Cell Biology; Cell culture; Cell isolation; Neuroscience.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Brain Stem Neoplasms / pathology*
Breeding
Disease Models, Animal
Genetic Engineering / methods*
Glioma / pathology*
Humans
Mice, Inbred C57BL
Mice, Transgenic
Neoplasm Grading
Spheroids, Cellular / pathology
Transposases / metabolism
Tumor Cells, Cultured

Substances

Transposases

Abstract

Publication types

MeSH terms

Substances

Grants and funding