Production of lipids by Chaetoceros affinis in media based on palm oil mill effluent

J Biotechnol. 2021 Feb 10:327:86-96. doi: 10.1016/j.jbiotec.2020.12.021. Epub 2021 Jan 6.

Abstract

Biomass and lipid production by the marine diatom Chaetoceros affinis were characterized under continuous light with aeration. Media based on palm oil mill effluent (POME; 10, 20 and 30 % v/v in distilled water) were used together with a standard control medium. The maximum biomass concentration on day 12 of batch cultures in control medium was 821 ± 71 mg L-1. Under identical conditions, in the best POME medium (20 % POME v/v in distilled water with other inorganic components), the biomass concentration was reduced by ∼11 % to 734 ± 66 mg L-1. The lipid content of the biomass grown in the control medium was 50.8 ± 4.5 % by dry weight, but was a little lower (48.9 ± 4.1 % by dry wt) in the above specified best POME medium. In the best POME medium, oleic acid was the major fatty acid (72.3 ± 5.2 % by weight) in the total lipids extracted from the biomass and monounsaturated fatty acids were the main type of fatty acids (74.6 ± 5.2 %). POME levels of >20 % in the medium suppressed both biomass and lipid production relative to the medium with 20 % POME.

Keywords: Algal lipids; Chaetoceros affinis; Diatom lipids; Lipid production; Palm oil mill effluent.

MeSH terms

  • Diatoms*
  • Industrial Waste* / analysis
  • Lipids
  • Palm Oil
  • Plant Oils

Substances

  • Industrial Waste
  • Lipids
  • Plant Oils
  • Palm Oil