Next-generation sequencing yields complete mitochondrial genome assembly of peaceful betta fish, Betta imbellis (Teleostei: Osphronemidae)

Syed Farhan Ahmad; Nararat Laopichienpong; Worapong Singchat; Aorarat Suntronpong; Tavun Pongsanarm; Thitipong Panthum; Nattakan Ariyaraphong; Jakaphan Bulan; Tanawat Pansrikaew; Kornsuang Jangtarwan; Navapong Subpayakom; Sahabhop Dokkaew; Narongrit Muangmai; Prateep Duengkae; Kornsorn Srikulnath

doi:10.1080/23802359.2020.1841582

Next-generation sequencing yields complete mitochondrial genome assembly of peaceful betta fish, Betta imbellis (Teleostei: Osphronemidae)

Mitochondrial DNA B Resour. 2020 Dec 24;5(4):3856-3858. doi: 10.1080/23802359.2020.1841582.

Authors

Syed Farhan Ahmad^{1

2}, Nararat Laopichienpong^{1

2}, Worapong Singchat^{1

2}, Aorarat Suntronpong^{1

2}, Tavun Pongsanarm^{1

2}, Thitipong Panthum^{1

2}, Nattakan Ariyaraphong^{1

2}, Jakaphan Bulan^{1

2}, Tanawat Pansrikaew^{1

2}, Kornsuang Jangtarwan^{1

2}, Navapong Subpayakom^{1

2}, Sahabhop Dokkaew³, Narongrit Muangmai^{2

4}, Prateep Duengkae², Kornsorn Srikulnath^{1

2

5

6

7}

Affiliations

¹ Department of Genetics, Faculty of Science, Laboratory of Animal Cytogenetics and Comparative Genomics (ACCG), Kasetsart University, Bangkok, Thailand.
² Department of Forest Biology, Faculty of Forestry, Special Research Unit for Wildlife Genomics (SRUWG), Kasetsart University, Bangkok, Thailand.
³ Department of Aquaculture, Faculty of Fisheries, Kasetsart University, Bangkok, Thailand.
⁴ Department of Fishery Biology, Faculty of Fisheries, Kasetsart University, Bangkok, Thailand.
⁵ Center for Advanced Studies in Tropical Natural Resources, National Research University-Kasetsart University (CASTNAR, NRU-KU), Kasetsart University, Bangkok, Thailand.
⁶ Center of Excellence on Agricultural Biotechnology (AG-BIO/PERDO-CHE), Bangkok, Thailand.
⁷ Amphibian Research Center, Hiroshima University, Higashihiroshima, Japan.

Abstract

The complete mitochondrial genome (mitogenome) of the peaceful betta (Betta imbellis) was obtained using next-generation sequencing. The sample of B. imbellis was collected from its native habitat in Southern Thailand. The mitogenome sequence was 16,897 bp in length, containing 37 genes with identical order to most teleost mitogenomes. Overall nucleotide base composition of the complete mitogenome was determined as AT bias. Phylogenetic analysis of B. imbellis showed a closer relationship with bubble-nesting fighting fish. This annotated mitogenome reference can be utilized as a bioresource for phylogenetic studies to support betta conservation programs.

Keywords: Peaceful betta; bioresource; bubble-nesting fighting fish.

Grants and funding

This research was financially supported by a grant from the National Research Council of Thailand [NRCT/16/2563] (awarded to SFA, NL, WS, AS, TP, TP, NA, JB, TP, KJ, NS, SD, NM, PD, and KS), the Center for Advanced Studies in Tropical Natural Resources, National Research University-Kasetsart University (awarded to KS), the Thailand Research Fund [Nos. RSA6180075, PHD60I0014, and PHD60I0082] through a grant awarded to KS, WS, and AS, the Science Achievement Scholarship of Thailand [No. 5917400296] through a grant from the Office of the Higher Education Commission, Thailand awarded to NL, the Capacity Building of KU Students on Internationalization Program (KUCSI) awarded to NL and KS, a grant from the Graduate Scholarship Program of the Graduate School, Kasetsart University, Thailand awarded to TP and KS, and a Postdoctoral Researcher award at Kasetsart University awarded to SFA and KS.