Dopamine induces functional extracellular traps in microglia

Ishan Agrawal; Nidhi Sharma; Shivanjali Saxena; S Arvind; Debayani Chakraborty; Debarati Bhunia Chakraborty; Deepak Jha; Surajit Ghatak; Sridhar Epari; Tejpal Gupta; Sushmita Jha

doi:10.1016/j.isci.2020.101968

Dopamine induces functional extracellular traps in microglia

iScience. 2021 Jan 6;24(1):101968. doi: 10.1016/j.isci.2020.101968. eCollection 2021 Jan 22.

Authors

Affiliations

¹ Department of Bioscience and Bioengineering, Indian Institute of Technology Jodhpur, Jodhpur, Rajasthan 342037, India.
² Karolinska Institute and Scilifelab, Stockholm, Sweden.
³ Department of Computer Science and Engineering, Indian Institute of Technology Jodhpur, Jodhpur, Rajasthan 342037, India.
⁴ Department of Neurosurgery, All India Institute of Medical Sciences Jodhpur, Jodhpur, 342005 Rajasthan, India.
⁵ Department of Anatomy, All India Institute of Medical Sciences Jodhpur, Jodhpur, 342005 Rajasthan, India.
⁶ Department of Pathology, Tata Memorial Hospital, Mumbai, 400012 Maharashtra, India.
⁷ Department of Radiation Oncology, Tata Memorial Hospital, Mumbai, 400012, Maharashtra, India.

Abstract

Dopamine (DA) plays many roles in the brain, especially in movement, motivation, and reinforcement of behavior; however, its role in regulating innate immunity is not clear. Here, we show that DA can induce DNA-based extracellular traps in primary, adult, human microglia and BV2 microglia cell line. These DNA-based extracellular traps are formed independent of reactive oxygen species, actin polymerization, and cell death. These traps are functional and capture fluorescein (FITC)-tagged Escherichia coli even when reactive oxygen species production or actin polymerization is inhibited. We show that microglial extracellular traps are present in Glioblastoma multiforme. This is crucial because Glioblastoma multiforme cells are known to secrete DA. Our findings demonstrate that DA plays a significant role in sterile neuro-inflammation by inducing microglia extracellular traps.

Keywords: Cell Biology; Cellular Neuroscience; Immunology; Molecular Biology.