Prevalence and phylogenetic analysis of HTLV-1 in blood donors in Golestan Province, in the Northeast of Iran

Hossein Mehrabi Habibabadi; Masoud Parsania; Ali Akbar Pourfathollah; Asma Bahrami; Zohreh Sharifi

doi:10.1016/j.jviromet.2021.114073

Prevalence and phylogenetic analysis of HTLV-1 in blood donors in Golestan Province, in the Northeast of Iran

J Virol Methods. 2021 Apr:290:114073. doi: 10.1016/j.jviromet.2021.114073. Epub 2021 Jan 21.

Authors

Hossein Mehrabi Habibabadi¹, Masoud Parsania², Ali Akbar Pourfathollah³, Asma Bahrami¹, Zohreh Sharifi⁴

Affiliations

¹ Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.
² Department of Microbiology, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
³ Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran; Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
⁴ Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran. Electronic address: z.sharifi@ibto.ir.

PMID: 33485939
DOI: 10.1016/j.jviromet.2021.114073

Abstract

The human T-lymphotropic virus type 1 (HTLV-1) can cause ATL or TSP. This study evaluates the prevalence of HTLV-1 infection in blood donors in Golestan province. The study was conducted among 4226 blood donors and ELISA test was performed for the initial HTLV-1 screening. Reactive samples were confirmed by Western blot and Electrochemiluminescence tests. Then recalling donors with reactive results was done and genomic DNA from the new sample was extracted and tested using the Nested PCR method and phylogenetic analysis was performed. At first, 8 samples were reactive with ELISA test and 4 samples were confirmed with western blot, Electrochemiluminescence and Nested PCR tests.The sequences of isolates was related to the HTLV-1 virus and subtype a (cosmopolitan) subgroup A.The prevalence of HTLV-1 virus in Golestan province was about 0.09 %.The genotype of virus isolates had a common ancestor with isolates of the Khorasan region.

Keywords: Golestan province; HTLV-1; Iran; Phylogenetic analysis; Prevalence.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Blood Donors*
HTLV-I Infections* / epidemiology
Human T-lymphotropic virus 1* / genetics
Humans
Iran / epidemiology
Phylogeny*
Prevalence