Quantification of drug-induced fractional killing using high-throughput microscopy

STAR Protoc. 2021 Jan 22;2(1):100300. doi: 10.1016/j.xpro.2021.100300. eCollection 2021 Mar 19.

Abstract

Anti-cancer drugs kill only a fraction of cells within a population at any given time. Here, we describe a protocol to quantify drug-induced fractional killing over time using high-throughput imaging. This protocol can be used to compare the effect of hundreds of conditions in parallel. We show how this protocol can be used to examine fractional killing in response to inhibitors of the mitogen-activated protein kinase pathway. For complete details on the use and execution of this protocol, please refer to Inde et al. (2020).

Keywords: Cancer; Cell biology; Cell-based assays; High-throughput screening; Microscopy.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Antineoplastic Agents / pharmacology*
  • Apoptosis / drug effects*
  • Cell Line, Tumor
  • Drug Screening Assays, Antitumor
  • Humans
  • MAP Kinase Signaling System / drug effects*
  • Neoplasms* / drug therapy
  • Neoplasms* / metabolism
  • Neoplasms* / pathology

Substances

  • Antineoplastic Agents