Cytosolic and Mitochondrial Ca2+ Imaging in Drosophila Dopaminergic Neurons

Tsuyoshi Inoshita; Yuzuru Imai

doi:10.1007/978-1-0716-1495-2_20

Cytosolic and Mitochondrial Ca²⁺ Imaging in Drosophila Dopaminergic Neurons

Methods Mol Biol. 2021:2322:207-214. doi: 10.1007/978-1-0716-1495-2_20.

Authors

Tsuyoshi Inoshita¹, Yuzuru Imai^{2

3}

Affiliations

¹ Department of Neurodegenerative and Demented Disorders, Juntendo University Graduate School of Medicine, Tokyo, Japan.
² Department of Research for Parkinson's Disease, Juntendo University Graduate School of Medicine, Tokyo, Japan. yzimai@juntendo.ac.jp.
³ Department of Neurology, Juntendo University School of Medicine, Tokyo, Japan. yzimai@juntendo.ac.jp.

PMID: 34043206
DOI: 10.1007/978-1-0716-1495-2_20

Abstract

The ATP-producing organelle mitochondrion controls cellular or synaptic Ca²⁺ concentrations through temporal uptake of Ca²⁺ outside of the mitochondria. Although intracellular Ca²⁺ influx occurs during neuronal activity, a persistently higher concentration of intracellular Ca²⁺ is neurotoxic. Healthy mitochondria ensure rapid Ca²⁺ uptake, which is necessary for proper neuronal activity. Mitochondrial Ca²⁺ buffering activity decreases in aged or sick neurons. In this chapter, we will introduce our protocol for evaluating Ca²⁺ buffering activity through the mitochondria during neuronal activity of dopaminergic neurons.

Keywords: Ca2+ buffering; Dopaminergic neuron; Drosophila; GCaMP; Live imaging; Mitochondria; Synaptic activity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Calcium / metabolism*
Calcium Signaling / physiology*
Cytosol / metabolism*
Dopaminergic Neurons / metabolism*
Drosophila / metabolism*
Mitochondria / metabolism*

Substances

Calcium