3D Optical Cryo-Imaging Method: A Novel Approach to Quantify Renal Mitochondrial Bioenergetics Dysfunction

Methods Mol Biol. 2021:2276:259-270. doi: 10.1007/978-1-0716-1266-8_20.

Abstract

Mitochondrial dysfunction contributes to various injuries and diseases. A mechanistic understanding of how dysfunctional mitochondria modulates metabolism is of paramount importance. Three-dimensional (3D) optical cryo-imager is a custom-designed device that can quantify the volumetric bioenergetics of organs in small animal models. The instrument captures the autofluorescence of bioenergetics indices (NADH and FAD) from tissues at cryogenic temperature. The quantified redox ratio (NADH/FAD) is used as an optical indicator of mitochondrial redox state.

Keywords: Bioenergetics; Fluorescence imaging; Mitochondria; Optical imaging; Redox state.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cryopreservation
  • Energy Metabolism
  • Flavin-Adenine Dinucleotide / analysis*
  • Flavin-Adenine Dinucleotide / metabolism
  • Frozen Sections
  • Imaging, Three-Dimensional / methods*
  • Kidney / chemistry*
  • Kidney / metabolism
  • Kidney / pathology
  • Mitochondria / chemistry*
  • Mitochondria / metabolism
  • Mitochondria / pathology
  • NAD / analysis*
  • NAD / metabolism
  • Optical Imaging / methods*
  • Oxidation-Reduction

Substances

  • NAD
  • Flavin-Adenine Dinucleotide