In Vivo Assessment of Mitochondrial Oxygen Consumption

Floor A Harms; Egbert G Mik

doi:10.1007/978-1-0716-1270-5_12

In Vivo Assessment of Mitochondrial Oxygen Consumption

Methods Mol Biol. 2021:2277:175-185. doi: 10.1007/978-1-0716-1270-5_12.

Authors

Floor A Harms¹, Egbert G Mik²

Affiliations

¹ Department of Anesthesiology, Laboratory of Experimental Anesthesiology, Erasmus MC - University Medical Center Rotterdam, Rotterdam, The Netherlands.
² Department of Anesthesiology, Laboratory of Experimental Anesthesiology, Erasmus MC - University Medical Center Rotterdam, Rotterdam, The Netherlands. e.mik@erasmusmc.nl.

PMID: 34080152
DOI: 10.1007/978-1-0716-1270-5_12

Abstract

The Protoporphyrin IX-Triplet State Lifetime Technique (PpIX-TSLT) has been proposed by us as a potential clinical noninvasive tool for monitoring mitochondrial function. We have been working on the development of mitochondrial respirometry for monitoring mitochondrial oxygen tension (mitoPO₂) and mitochondrial oxygen consumption (mitoVO₂) in skin. In this work, we describe the principles of the method in small experimental animals.

Keywords: Mitochondrial oxygen consumption (mitoVO2); Mitochondrial oxygen tension (mitoPO2); Oxygen-dependent quenching; Phosphorescence quenching; Tissue oxygenation.

MeSH terms

Aminolevulinic Acid / pharmacology
Animals
Body Temperature
Equipment Design
Luminescent Measurements / instrumentation
Luminescent Measurements / methods
Mitochondria / metabolism*
Oxygen Consumption*
Protoporphyrins / chemistry
Rats
Rats, Wistar
Respiration, Artificial
Skin / drug effects
Tracheotomy

Substances

Protoporphyrins
Aminolevulinic Acid
protoporphyrin IX