Assessment of Mitochondrial Reactive Oxygen Species and Redox Regulation in Stem Cells

Methods Mol Biol. 2021:2277:289-297. doi: 10.1007/978-1-0716-1270-5_18.

Abstract

Mitochondrial reactive oxygen species (mtROS) and redox regulation play an important role in stem cell maintenance and cell fate decisions. Although changes in mtROS and redox homeostasis represent a physiological mechanism to drive stem cell commitment and differentiation, dysregulation of this system can lead to defects in stem cell maintenance and regenerative capacity. This chapter explains the methods used to assess mitochondrial superoxide levels and redox regulation in stem cell populations.

Keywords: Antioxidant; Electron transport chain; Metabolism; Mitochondria; Oxidative stress; Reactive oxygen species (ROS); Redox; Stem cell fate; Stem cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Gene Expression Profiling / methods
  • Mice
  • Mitochondria / metabolism*
  • Muscle, Skeletal / cytology
  • Organophosphorus Compounds / chemistry
  • Oxidation-Reduction
  • Phenanthridines / chemistry
  • Reactive Oxygen Species / analysis*
  • Reactive Oxygen Species / metabolism
  • Stem Cells / metabolism*
  • Stem Cells / physiology
  • Superoxide Dismutase / genetics
  • Superoxides / analysis
  • Superoxides / metabolism
  • Uncoupling Protein 2 / genetics

Substances

  • MitoSox Red
  • Organophosphorus Compounds
  • Phenanthridines
  • Reactive Oxygen Species
  • Ucp2 protein, mouse
  • Uncoupling Protein 2
  • Superoxides
  • Superoxide Dismutase
  • superoxide dismutase 2