Further studies on the resolution of human mercapt- and nonmercaptalbumin and on human serum albumin in the elderly by high-performance liquid chromatography

S Era; T Hamaguchi; M Sogami; K Kuwata; E Suzuki; K Miura; K Kawai; Y Kitazawa; H Okabe; A Noma

doi:10.1111/j.1399-3011.1988.tb00900.x

Further studies on the resolution of human mercapt- and nonmercaptalbumin and on human serum albumin in the elderly by high-performance liquid chromatography

Int J Pept Protein Res. 1988 May;31(5):435-42. doi: 10.1111/j.1399-3011.1988.tb00900.x.

Authors

S Era¹, T Hamaguchi, M Sogami, K Kuwata, E Suzuki, K Miura, K Kawai, Y Kitazawa, H Okabe, A Noma, et al.

Affiliation

¹ Department of Physiology, School of Medicine, Gifu University, Japan.

PMID: 3410634
DOI: 10.1111/j.1399-3011.1988.tb00900.x

Abstract

High-performance liquid chromatographic (HPLC) analysis of human serum albumin (HSA) on Asahipak GS-520 showed at least two peaks, the principal component corresponding to human mercaptalbumin (HMA) and the secondary one to nonmercaptalbumin (HNA). HPLC analysis of HSA on Asahipak ES-520 N showed three peaks, the principal component corresponding to HMA, the secondary one to HNA having mixed disulfide with cysteine or glutathione and the tertiary one to HNA oxidized higher than mixed disulfide. Two kinds of rapid HPLC for the resolution of HSA into HMA and HNA were developed by the present authors. Using these HPLC, the present authors found a significant decrease in the fraction of HMA in the elderly.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aged
Chromatography, High Pressure Liquid
Disulfides / analysis
Humans
Reference Values
Serum Albumin / isolation & purification*

Substances

Disulfides
Serum Albumin
mercaptoalbumin