Concurrent recordings of hippocampal neuronal spikes and prefrontal synaptic inputs from an awake rat

Yuya Nishimura; Yuji Ikegaya; Takuya Sasaki

doi:10.1016/j.xpro.2021.100572

Concurrent recordings of hippocampal neuronal spikes and prefrontal synaptic inputs from an awake rat

STAR Protoc. 2021 Jun 8;2(2):100572. doi: 10.1016/j.xpro.2021.100572. eCollection 2021 Jun 18.

Authors

Yuya Nishimura¹, Yuji Ikegaya^{1

2

3}, Takuya Sasaki^{1

4}

Affiliations

¹ Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
² Center for Information and Neural Networks, National Institute of Information and Communications Technology, 1-4 Yamadaoka, Suita City, Osaka 565-0871, Japan.
³ Institute for AI and Beyond, The University of Tokyo, Tokyo 113-0033, Japan.
⁴ Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency (JST), 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan.

Abstract

A major challenge in neuroscience is linking synapses to cognition and behavior. Here, we developed an experimental technique to concurrently conduct a whole-cell recording of a prefrontal neuron and a multiunit recording of hippocampal neurons from an awake rat. This protocol includes surgical steps to establish a cranial window and 3D printer-based devices to hold the rat. The data sets allow us to directly compare how subthreshold synaptic transmission is associated with suprathreshold spike patterns of neuronal ensembles. For complete details on the use and execution of this protocol, please refer to Nishimura et al. (2021).

Keywords: Behavior; Cell Biology; Neuroscience.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Action Potentials*
Animals
Hippocampus / physiology*
Patch-Clamp Techniques
Prefrontal Cortex / physiology*
Rats
Synapses / physiology*
Wakefulness