Application of FluoVolt Membrane Potential Dye for Induced Pluripotent Stem Cell-Derived Cardiac Single Cells and Monolayers Differentiated via Embryoid Bodies

Methods Mol Biol. 2021:2320:101-110. doi: 10.1007/978-1-0716-1484-6_11.

Abstract

FluoVolt, a membrane potential dye, has been used to depict the action potentials of cardiomyocytes derived from human-induced pluripotent stem cells (hiPSC-CMs) in order to classify the cardiac cell subtype, evaluate long QT syndrome, and conduct cardiotoxic drug-responsive tests. To apply FluoVolt, users must prepare the hiPSC-CMs, assess the dye loadings, and apply the excitation light. Here we describe the steps to measure action potentials from single hiPSC-CMs and hiPSC-CM monolayers using this dye.

Keywords: Action potential; Cardiac cell subtype; Cardiac single cells; FluoVolt; Induced pluripotent stem cell; Membrane potential dye.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Action Potentials / physiology*
  • Cell Differentiation / physiology*
  • Cells, Cultured
  • Coloring Agents / chemistry*
  • Embryoid Bodies / cytology*
  • Humans
  • Induced Pluripotent Stem Cells / cytology*
  • Long QT Syndrome / therapy
  • Myocytes, Cardiac / cytology*

Substances

  • Coloring Agents