Abstract
Astrocytes play important roles in neurodevelopment and diseases. Previous studies described ways to derive astrocytes from somatic cells by going through iPSC or iNSC/iNPC intermediates. Here we describe a method to directly convert mouse fibroblasts into functional astrocytes using small molecules without transgenes or viral transduction. The direct chemical reprogramming method described in this study provides a more rapid way to derive astrocytes from fibroblasts.
Keywords:
Chemical reprogramming; Direct conversion; Induced astrocytes; Small molecules; iPSC-derived astrocytes; iPSCs.
© 2021. Springer Science+Business Media, LLC, part of Springer Nature.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Astrocytes / cytology*
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Astrocytes / drug effects*
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Astrocytes / metabolism
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Biomarkers
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Cell Differentiation / drug effects*
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Cell Differentiation / genetics
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Cell Separation
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Cells, Cultured
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Cellular Reprogramming / drug effects*
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Cellular Reprogramming / genetics
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Cellular Reprogramming Techniques*
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Fibroblasts / cytology*
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Fibroblasts / drug effects*
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Fibroblasts / metabolism
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Gene Expression
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Humans
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Immunohistochemistry
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Induced Pluripotent Stem Cells / cytology
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Induced Pluripotent Stem Cells / drug effects
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Induced Pluripotent Stem Cells / metabolism
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Mice
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Transcription Factors / genetics
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Transgenes
Substances
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Biomarkers
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Transcription Factors