Lipidomics dataset of Danio rerio optic nerve regeneration model

Jennifer Arcuri; Matthew B Veldman; Sanjoy K Bhattacharya

doi:10.1016/j.dib.2021.107260

Lipidomics dataset of Danio rerio optic nerve regeneration model

Data Brief. 2021 Jul 14:37:107260. doi: 10.1016/j.dib.2021.107260. eCollection 2021 Aug.

Authors

Jennifer Arcuri^{1

2

3}, Matthew B Veldman⁴, Sanjoy K Bhattacharya^{1

2

3}

Affiliations

¹ Bascom Palmer Eye Institute, Miller School of Medicine at University of Miami, Miami, FL, 33136, USA.
² Miami Integrative Metabolomics Research Center, Miami, FL, 33136, USA.
³ Molecular Cellular Pharmacology Graduate Program, University of Miami, Miami, FL, 33136, USA.
⁴ Department of Cell Biology, Neurobiology, and Anatomy, Medical College of Wisconsin, Milwaukee, WI 53226, USA.

Abstract

The right optic nerve of adult, 6 month to 1 year old, female and male Danio rerio were crushed and collected three days after. Matching controls of uninjured left optic nerves were also collected. The tissue was dissected from euthanized fish and frozen on dry ice. Samples were pooled for each category (female crush, female control, male crush, male control) n = 24 to obtain sufficient tissue for analysis. The brain from one male fish was also collected for control/calibration. Lipid extraction was done with the Bligh and Dyer [1] method, followed by untargeted liquid chromatography-mass spectrometry (LC MS-MS) lipid profiling using a Q-Exactive Orbitrap instrument coupled with Vanquish Horizon Binary UHPLC LC-MS system. The lipids were identified and quantified with LipidSearch 4.2.21 and the statistical analysis was conducted through Metaboanalyst 5.0. This data is available at Metabolomics Workbench, Study ID ST001725.

Keywords: CNS Injury; Lipids; Optic Nerve; Regeneration; Zebra-fish.